http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112064121-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6f0ff925e8faf2436b486a4701fac48 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 |
| filingDate | 2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c547bd71ee87e1a6ab13aa0a227e860d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_daf7f005682249259ae5fcd5fd8b256c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_872229529a51eb156685ac0e355d350f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_356ce1b4c88aead447aa9e7c036fa8d1 |
| publicationDate | 2020-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112064121-A |
| titleOfInvention | Multiple PCR sequencing method for collecting RNA reverse transcription, enriching and establishing library and library establishing instrument |
| abstract | The invention provides a multiplex PCR sequencing method integrating RNA reverse transcription and enrichment and library building, which comprises the following steps: selecting a genome locus or an RNA region as an RNA template and carrying out primer design; carrying out reverse transcription and first PCR amplification on the RNA template to obtain a first product; carrying out first purification treatment on the first product to obtain a second product; adding the primer group into the second product to perform second PCR amplification to obtain a third product; and carrying out second purification treatment on the third product to finish library construction and enrichment. In addition, the invention can also be applied to an automatic library building instrument. The technical scheme provided by the invention combines reverse transcription and multiple PCR amplification into one step, RNA library establishment and enrichment are rapid, simple and convenient, the RNA library establishment and enrichment time is shortened, and the library establishment efficiency is further improved. |
| priorityDate | 2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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