http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112063607-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 |
| filingDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112063607-B |
| titleOfInvention | Nitrilase mutant and application thereof in catalytic synthesis of 2-chloronicotinic acid |
| abstract | The invention discloses a nitrilase mutant and application thereof in catalytic synthesis of 2-chloronicotinic acid, belonging to the technical field of enzyme engineering. The amino acid sequence of the nitrilase mutant is shown in SEQ ID NO.4, namely, the 167 th tryptophan W of the parent nitrilase is mutated into the glycine G. The nitrile hydrolysis mutant provided by the invention eliminates the hydration activity of parent nitrilase to 2-chloronicotinonitrile, generates no byproduct 2-chloronicotinamide in the catalysis process, greatly improves the nitrile hydrolysis activity, can specifically catalyze the hydrolysis of 2-chloronicotinonitrile to synthesize 2-chloronicotinic acid, and has important potential in the enzymatic industrial synthesis of 2-chloronicotinic acid. |
| priorityDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 312.