http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112062852-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-20 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 |
filingDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112062852-B |
titleOfInvention | Variable region sequence of anti-clothianidin and dinotefuran broad-spectrum antibody and preparation of recombinant complete antibody thereof |
abstract | The invention discloses a variable region sequence of an anti-clothianidin and dinotefuran broad-spectrum antibody, wherein the amino acid sequence of a heavy chain variable region coding gene is shown in SEQ ID NO. 2. The invention also discloses an anti-clothianidin and dinotefuran broad-spectrum recombinant complete antibody, which comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein the amino acid sequence of a heavy chain variable region coding gene is shown as SEQ ID NO. 2. The sequence genes obtained by the invention are respectively connected to expression vectors containing heavy chain constant region genes and light chain constant region genes, and the recombinant complete antibody is obtained by adopting mammalian cell expression of a dual-plasmid system, so that the recognition activity of the murine parent antibody is ensured, the anti-clothianidin and dinotefuran broad-spectrum antibody can be stably produced in large batch, and reliable core reagents are provided for various immunoassay methods for synchronously detecting clothianidin and dinotefuran. |
priorityDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 177.