http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112051354-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N31-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-10 |
| filingDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112051354-B |
| titleOfInvention | Lipase determination kit and preparation method thereof |
| abstract | The invention discloses a lipase determination kit and a preparation method thereof, wherein an R1 reagent comprises a buffer solution, sodium deoxycholate, colipase, a metal salt, a protective agent, a surfactant and a preservative; the R2 reagent comprises L-tartaric acid, sodium taurodeoxycholate, 1, 2-o-dilauryl-racemic-glycerol-3-pentanoic acid- (6-methyl resorufin) ester, a cosolvent, a stabilizer and a preservative, wherein the cosolvent comprises ethyl acetate, dilauryl glycerol sulfate and a Triton surfactant. According to the invention, ethyl acetate, dilauryl glycerol sulfate and Triton surfactant are preferably used as cosolvents together to exert the synergistic interaction of the three, so that the solubility of the substrate is greatly improved, and furthermore, mercaptoethanol and mannitol are used as composite stabilizers, so that the lipase determination kit with good stability, high accuracy and precision and remarkably improved linear range is obtained. |
| priorityDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.