http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112048517-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43563 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P7-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
filingDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112048517-B |
titleOfInvention | Transgenic biocontrol fungus for interfering diaphorina citri vitellogenin gene expression and preparation method and application thereof |
abstract | The RNAi vector comprises a skeleton vector and a hairpin structure, wherein the hairpin structure is an ihpRNA expression box, the structure of the ihpRNA expression box is a PtrpC promoter, a forward target gene fragment, an intron, a reverse target gene fragment and a TtrPC terminator structure, and the target gene fragment is a diaphorina vitellogenin gene conserved sequence DcVg1a or DcVg1 b; the skeleton vector is a filamentous fungus expression vector. Also discloses a transgenic biocontrol fungus and a preparation method and application thereof, wherein the transgenic biocontrol fungus is obtained by adopting the RNAi vector to transform the biocontrol fungus. Experiments prove that the obtained transgenic Lecanicillium wilsonii can effectively improve the toxicity to the diaphorina citri and shorten the impregnation time to the diaphorina citri. |
priorityDate | 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 282.