| abstract |
The invention discloses a 4-chlorophenoxyacetic acid hapten, an artificial antigen and its application in immune detection. The arm introduced into the 4-chlorophenoxyacetic acid hapten designed by the present invention not only has an active group, but also can completely retain the carboxyl group of the target to be detected, so that the electron cloud density of the hapten and the target to be detected is consistent, and even Simple to connect. The 4-chlorophenoxyacetic acid artificial antigen and monoclonal antibody constructed based on this have strong specificity for ELISA detection, and the IC50 value is 10.9μg/L; when used in colloidal gold immunochromatography, the sensitivity is 20μg/L, and the actual sample The detection limit was 70 μg/kg, the false negative and false positive rates were 0, and it had good specificity and stability. |