http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112033783-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_083fc14921e49c9c51f27e15ca6ff3d7 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f94b1dabb4f66bc886f0e692267d6397 |
| publicationDate | 2020-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112033783-A |
| titleOfInvention | DNA ploid staining solution, preparation method and staining method |
| abstract | The invention relates to the field of biotechnology, in particular to a DNA ploid staining solution, a preparation method and a staining method, wherein the prepared DNA staining solution can solve the problems that the background is easy to be co-stained, the staining time is long, and the section is easy to fade in the process of slide preparation, so that a reliable histochemical staining technology is provided for the image determination of the DNA content of tumor cell nuclei, the cell nuclei are fixed by using alcohol with the concentration of 95 percent, the phenomenon that the DNA forms small fragments and is dissociated from the cell nuclei in the process of staining hydrolysis to influence the accuracy of DNA measurement is avoided, aldehyde groups generated before hydrolysis can be sealed by fixing, and false positive products are reduced. Meanwhile, the BS fixing solution can change the space structure of the DNA molecules, influence the sensitivity to acid hydrolysis, and avoid the phenomenon that chromatin is slightly colored due to the large difference of the intensity of the staining reaction caused by improper selection of the fixing solution or overlong tissue fixing time. The DNA staining result is clear and the background is cleaner by gradient alcohol dehydration. |
| priorityDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.