http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112029769-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0337 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61D19-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61D19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89 |
| filingDate | 2020-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112029769-B |
| titleOfInvention | Construction method of Cyp1a1 gene knockout mouse model and application of model in sepsis |
| abstract | The invention discloses a construction method of a Cyp1a1 gene knockout mouse model and application thereof in sepsis, wherein the construction method comprises the following steps: determining a target site of a gene to be knocked out of a mouse, and designing sgRNA, wherein the sequence of the sgRNA is shown as SEQ ID No. 2-5; co-injecting or co-electrotransforming the active sgRNA and Cas9 mRNA or Cas9 Protein into mouse fertilized egg cells, transplanting the fertilized eggs into a receptor mother mouse for inoculation, and obtaining an F0 generation gene knockout mouse; f0 generation mice and wild mice are hybridized to obtain F1 generation Cyp1a1 gene knockout mice. The invention successfully prepares the Cyp1a1 gene knockout mouse model, applies the model to the research of sepsis pathological process for the first time, and unexpectedly finds that the bacterial removing capability of the Cyp1a1 gene knockout mouse is obviously enhanced, the survival capability is improved when the mouse deals with endotoxemia and gram-negative bacteria attack, and the anti-infection capability is enhanced. The invention also applies the Cyp1a1 gene knockout mouse to the metabolic regulation research of sepsis for the first time, and provides a reliable animal model for the research of the relevant metabolic mechanism in the critical illness field. |
| priorityDate | 2020-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 146.