| abstract |
The invention relates to a large-scale culture method of helicobacter pylori, which utilizes a helicobacter pylori liquid-solid culture device and comprises the following steps: (A) coating the stirrer with 1mg/ml fibrinogen; (B) preparing a solid culture medium; (C) helicobacter pylori is prepared into 1 × 10 9 Injecting the bacterium solution per ml into a pylorus screw bacterium solution-solid culture device, coating bacteria, and culturing with a solid culture medium; (D) preparing a liquid culture medium; (E) culturing helicobacter pylori in solid culture medium for 12 hr, injecting liquid culture medium, culturing in liquid culture medium for 12 hr, stirring, culturing for 1-2 days, discharging liquid culture medium, and collecting helicobacter pylori. The invention has short culture period, simple operation and more helicobacter pylori, and provides basic conditions for preparing inactivated whole-bacterium vaccine. |