http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112014572-A

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filingDate 2020-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93a9d9b179f66d73722bcf01a17e7394
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publicationDate 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112014572-A
titleOfInvention A kind of latex particle preparation method for detecting KL-6 and its application
abstract The invention discloses a preparation method for detecting KL-6 latex particles, and relates to the technical field of biological analysis, comprising (1) suspending latex microspheres with a reaction buffer to obtain a latex microsphere solution; (2) reacting to step (1) The KL-6 antibody is added to the system; (3) the reaction is continuously stirred; (4) EDC.HCl solution and NHS solution are added to the reaction system of step (3) in turn; (5) a blocking solution is added to the reaction system of step (4), Centrifuge after the reaction; (6) add a storage solution to the reaction system in step (5); (7) store overnight to obtain a latex reagent. The beneficial effects of the present invention are that the prepared latex particles can effectively improve the sensitivity and specificity when the latex particles are used for the latex-enhanced immune turbidimetric determination of KL-6, and have strong anti-interference properties, and can effectively reduce interfering substances, such as bilirubin Interference of hormones, hemoglobin, triglycerides, ascorbic acid, fat emulsions, rheumatoid factor.
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priorityDate 2020-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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