| abstract |
The present invention relates to the optimized expression and preparation of light-sensitive channel ChR2 protein. Specifically, the present invention has carried out a targeted optimization design on the coding sequence of the light-sensitive channel ChR2 protein, constructed a recombinant expression vector of the light-sensitive channel ChR2 protein, and put the optimized light-sensitive channel ChR2 protein coding sequence (SEQ ID NO:2 ) into host cells, the expression of light-sensitive channel ChR2 protein is very high, and the photosensitive ability is strong, which is very suitable for expression in mammalian (especially human) cells. |