| abstract |
The invention discloses a creatine amidinohydrolase mutant with improved activity, which belongs to the technical field of enzyme engineering. The present invention uses a consensus method without phylogenetic bias to analyze the sequence of creatine amidinohydrolase, screens out a single-point mutant with significantly improved activity, and performs site-directed mutation on it to obtain mutant enzymes D17V/K351E and T199S with improved activity /K351E, D17V/T199S/K351E, D17V/T117P/K351E compared with the wild type, the activity of these mutant enzymes increased by about 2 times. |