http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111996178-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01009 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111996178-B |
| titleOfInvention | A kind of histidine alcohol phosphate aminotransferase mutant, engineering bacteria and application |
| abstract | The invention discloses a histidine alcohol phosphate aminotransferase mutant, engineering bacteria and applications, and belongs to the field of biotechnology. The histidine alcohol phosphate aminotransferase gene is derived from the strain HY61 of Streptomyces garcinata, and its nucleotide sequence is shown in SEQ ID NO: 1, and its amino acid sequence is shown in SEQ ID NO: 2. The mutant is obtained by single mutation or multi-site mutation at the 66th, 138th, 197th, 226th, and 311th positions of the amino acid sequence shown in SEQ ID NO: 2. The histidine alcohol phosphate aminotransferase mutant engineering strain constructed by the invention can effectively improve the stability of 3-indolepyruvic acid with the use of nitrogen filling protection and stabilizer, the product concentration can reach up to 179.8mM, and the conversion rate is greater than 89%, and the raw materials are cheap and easy to obtain, the production process is simple and feasible, and the production cost is low, and high-yield green production of 3-indolepyruvic acid is realized. |
| priorityDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 218.