http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111983230-A

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filingDate 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5b91dc4b3c261789d2af3b8aa6eaaac
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publicationDate 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111983230-A
titleOfInvention Immunoassay kit for detecting IgG4 receptor of M-type phospholipase A2 and detection method thereof
abstract The invention relates to a time-resolved fluorescence immunoassay method for simultaneously detecting autoantibodies IgG4 of different epitope determinants of an M-type phospholipase A2 receptor (PLA2R), belonging to the field of clinical immunology detection. Respectively coating purified recombinant antigens of different epitope determinants of PLA2R on a microporous plate, adding diluted serum of a patient to be tested, and adding Eu 3+ Anti-human IgG4 antibody, no Eu attached after the end of the labeling immunoreaction 3+ Anti-human IgG4 antibody will be washed away. After adding the enhancement liquid, respectively detecting Eu by measuring with a time-resolved fluorometer 3+ The concentration of IgG4 antibody of different epitopes of PLA2R in the sample is obtained according to a standard curve. The kit related by the invention can simultaneously detect the content of different epitope determinants IgG4 of PLA2R, and provides convenience for clinical application.
priorityDate 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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