http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111983112-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b1cdf57c05fd6ba81bede23efb605f3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2020-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3e20e741abd14742f12b8f6bd62da88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c638928152bee6df1d3d1d5110d86b8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6240bfcd4fe3b51488914cf0b30d7e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00a2acbdd8bfee8844f5ac6f29b24bd4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e2da28f60c506c95df909e12c6773ab |
| publicationDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111983112-A |
| titleOfInvention | Detection method of TMAO and its related metabolites in serum |
| abstract | The invention provides a detection method of TMAO and its related metabolites in serum. The method includes: S1, preparing a standard solution of TMAO and its related metabolites; S2, selecting two deuterated compounds as internal standards, and preparing an internal standard solution with a protein precipitant and an internal standard; S3, combining the standard solution with the internal standard The standard solution is mixed to obtain the standard solution to be tested; the standard solution to be tested is sequentially separated by ultra-high performance liquid chromatography and detected by mass spectrometry to obtain a standard curve; S4, the serum to be tested and the internal standard solution are mixed, centrifuged, and the supernatant is taken The liquid is used as the sample to be tested; S5, the sample to be tested is sequentially separated by ultra-high performance liquid chromatography and detected by mass spectrometry, and compared with the standard curve to obtain the contents of TMAO and its related metabolites in the serum to be tested respectively. The invention realizes the absolute quantification of TMAO and its related metabolites, has strong specificity, high sensitivity, short running time, simple and quick operation. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114942276-A |
| priorityDate | 2020-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.