http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979351-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5fe74d097331a8f9907dec37e6a28aa |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-706 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2019-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97798a6e34c08859e0e4c8d3b75648db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83bf8ef69be7f119738a2d82e16f70b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a498b7239c38c36c30004a48e1e592c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb5e2cd7348ac3d9bd5c75e90394d4d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3891826c1361f09b861d2e3f82f6d723 |
| publicationDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111979351-A |
| titleOfInvention | Hepatitis B virus nucleic acid quantitative detection method and kit |
| abstract | The present invention provides a method for quantitative detection of hepatitis B virus nucleic acid. The specific steps include: using a hepatitis B virus-nucleic acid release agent to rapidly lyse and release the hepatitis B virus DNA in serum or plasma specimens; A pair of specific primers and a specific fluorescent probe designed in the conservative region are matched with PCR reaction solution. On the fluorescence quantitative PCR instrument, the real-time fluorescent quantitative PCR detection method is used to realize the quantification of hepatitis B virus DNA through the change of fluorescent signal. detection. The whole test process does not need to extract DNA from the samples separately, just add serum or plasma samples directly into the PCR reaction tube and mix thoroughly with the hepatitis B virus-nucleic acid release agent, which can be used as the template for PCR amplification, avoiding the routine sample nucleic acid extraction process. environmental pollution in the process, and the whole process takes a short time. The present invention also provides a kit for quantitative detection of hepatitis B virus nucleic acid, which is easy to measure and easy to operate. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250325-A |
| priorityDate | 2019-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.