http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111961724-A

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filingDate 2020-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1136ba5ce5cde0be73d5f72fadcbb573
publicationDate 2020-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111961724-A
titleOfInvention Pancreatic cancer detection kit and detection method (PCR method)
abstract The invention discloses a pancreatic cancer detection kit, which comprises PCR reaction liquid, polymerase, a negative control sample and a positive control sample, wherein the PCR reaction liquid comprises dNTPs, PCR Buffer, primers and probes, the volume and the number of the raw materials are respectively 0.96mLx2 parts, the polymerase is Taq DNA polymerase, and the volume and the number of the raw materials are respectively 85 mulx 1 parts. The invention relates to the technical field of genetic engineering, in particular to a pancreatic cancer detection kit and a detection method (PCR method), which can distinguish non-cancerous tissues and cancerous tissues of pancreas through DNA methylation state, wherein the sensitivity and specificity of cancer tissue diagnosis are both 100 percent, and can discover that acid Secreted Protein (SPARC) rich in cysteine of a relapse patient plays a role in regulating cell matrix and the aspects of tumor angiogenesis, hyperplasia and migration as soon as possible. SPARC gene methylation occurs in early pancreatic cancer, so that detection of SPARC gene methylation is very significant for early diagnosis of adenocarcinoma.
priorityDate 2020-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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