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filingDate 2020-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111961638-B
titleOfInvention An effective method for extracting plant mitochondria
abstract A method for effectively extracting plant mitochondria, the technical core of the method lies in differential centrifugation combined with inorganic alumina filter membrane, mainly for plant mesophyll cells, to efficiently separate and purify mitochondria, and the steps are: 1) culturing plant materials; 2) grinding Buffer preparation; 3) Sample preparation; 4) Needle syringe filter filtration; 5) Fluorescence microscopy to detect mitochondria. The invention has convenient operation and stable effect, and is of great significance for exploring the function of plant mitochondria.
priorityDate 2020-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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