http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111944865-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-0104 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-60 |
filingDate | 2020-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111944865-B |
titleOfInvention | Application of a bacterial-derived α-L-rhamnosidase in the efficient production of hesperetin-7-O-glucoside |
abstract | The invention relates to the application of a bacterial-derived α-L-rhamnosidase in the efficient production of hesperetin-7-O-glucoside. The nucleotide sequence of the α-L-rhamnosidase FjRha in the present invention is shown in SEQ ID NO. 1, and the gene is cloned from the genome of Flavobacterium johnsonii. The α-L-rhamnosidase FjRha can specifically hydrolyze artificial substrates and natural substrates containing α-1,6 rhamnoside bonds, and can efficiently and specifically catalyze hesperidin to prepare hesperetin-7-O glucoside, the conversion rate of catalyzing 3g/L of hesperidin to generate hesperetin-7-O-glucoside is up to more than 95% under the conditions of enzyme dosage 8U/mL, pH 7.0, and 37°C, and the method has simple steps. , low cost, mild conditions, environmental friendliness and other advantages, has broad application prospects. |
priorityDate | 2020-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 437.