http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111926030-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10021 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111926030-B |
| titleOfInvention | Phage genome editing vector based on CRISPR-Cas12a system and application thereof |
| abstract | The invention discloses a phage genome editing vector based on a CRISPR-Cas12a system, which comprises a LbCas12a protein coding frame nucleic acid sequence regulated by a proC promoter, a crRNA expression frame nucleic acid sequence regulated by a J23100 promoter, a plasmid framework nucleic acid sequence comprising an Escherichia coli replication factor CloDF13, a spectinomycin tolerance gene SMR and a proC promoter element responsible for regulating LbCas12a protein expression. The invention also discloses a construction method of the vector and application of the vector in phage genome editing. Many phages resist the cutting of host nuclease to the genome of the phages by carrying out covalent chemical modification on the self genome DNA (for example, cytosine in the T4 phage genome is highly glycosylated), and the self-protection mechanism of the phages brings great obstacles to genome editing. |
| priorityDate | 2020-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.