http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111926004-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4fb38ebd40c70e97cc5f1519c6699162 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2020-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c62b1ce90db7cf209852bdf659b8c9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbd1c0be364614033737a86b5274c05f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43a648bb6f45deeed6b5fa55fd5eeb6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_348344565584c2e4cff55af52259ba38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e3554d1cd0f885d410aeb63c12651e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_019301dc81c590482b60410b5add3a91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d76b89eb7850e96bf3b344568bf0d66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_653897f8f1b53d1c7616931212732bd8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d18e119a193c4c4fc5a464c38961f8ea |
| publicationDate | 2020-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111926004-A |
| titleOfInvention | Extraction method of macrovirome DNA from activated sludge |
| abstract | The invention relates to a method for extracting macrovirome DNA in activated sludge, comprising the following steps: (1) taking the deposited sludge, resuspending it in a potassium citrate buffer solution with a concentration of 0.8-1.5% by mass, 80-120W, 15-25kHz ultrasonic mixing for 2-5min to prepare bacterial liquid; (2) removing microbial impurities from the bacterial liquid obtained in step (1) to obtain an extract; (3) preparing an extract from step (2); After the obtained extract is concentrated, it is treated with DNaseI to obtain an extract for removing impurity DNA; (4) the virus protein capsid is removed from the extract obtained in step (3) for removing impurity DNA, and then DNA is extracted and purified. , the macrovirome DNA was prepared. The method of the invention can not only effectively extract the macroviral DNA of the activated sludge, but also greatly improves the yield and quality of the macroviral DNA, and has the advantages of simple operation, strong feasibility and low cost, and has broad application prospects. |
| priorityDate | 2020-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.