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filingDate 2019-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdd52e9e21bce3f089617b19c6409113
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publicationDate 2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111909931-A
titleOfInvention Fluorescent probe, primer pair, fluorescent quantitative PCR kit and detection method
abstract The present invention provides a fluorescent probe, the fluorescent probe includes a single-stranded nucleotide fragment, the 5' end of the single-stranded nucleotide fragment is labeled with a fluorescent reporter group, and the nucleotide fragment has a The 3' end is labeled with a fluorescence quenching group, and the single-stranded nucleotide fragment includes the nucleotide sequence shown in SEQ ID NO: 1. The fluorescent probe has the advantages of high specificity and high sensitivity, and the fluorescent probe can be used for rapid and quantitative analysis of the copy number of lentiviruses containing WPRE elements, especially CAR-T mediated by lentiviral genes containing WPRE elements Lentiviral copy number in . The invention also provides a primer pair, a fluorescent quantitative PCR kit and a detection method.
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