Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4aec56d5a039d93a130a2aabf6bd490f |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate |
2019-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdd52e9e21bce3f089617b19c6409113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd821fcf29e1a8f31c1e13065f9733d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b44bfa16234897310902f7f62eff18a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cea8e7d973e7fe2f6d2128a88c3145e5 |
publicationDate |
2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-111909931-A |
titleOfInvention |
Fluorescent probe, primer pair, fluorescent quantitative PCR kit and detection method |
abstract |
The present invention provides a fluorescent probe, the fluorescent probe includes a single-stranded nucleotide fragment, the 5' end of the single-stranded nucleotide fragment is labeled with a fluorescent reporter group, and the nucleotide fragment has a The 3' end is labeled with a fluorescence quenching group, and the single-stranded nucleotide fragment includes the nucleotide sequence shown in SEQ ID NO: 1. The fluorescent probe has the advantages of high specificity and high sensitivity, and the fluorescent probe can be used for rapid and quantitative analysis of the copy number of lentiviruses containing WPRE elements, especially CAR-T mediated by lentiviral genes containing WPRE elements Lentiviral copy number in . The invention also provides a primer pair, a fluorescent quantitative PCR kit and a detection method. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112575093-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112322711-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112481361-A |
priorityDate |
2019-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |