| abstract |
The invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method of snake venom thrombin. The quantitative detection method of the above-mentioned snake venom-like thrombin is as follows: take the characteristic polypeptide reference substance of the snake venom-like thrombin of the white-browed pit viper with the amino acid sequence of LDSPVSNSAHPLSLPSSAPSVGSVCR, and prepare a series of concentration reference substance solutions; The supernatant after the solution is used as a series of solutions to be tested; the solutions to be tested are respectively injected into a liquid chromatography-mass spectrometer, and qualitative ion pairs and quantitative ion pairs are selected to detect the content of characteristic polypeptides in the solutions to be tested. The invention adopts the standard addition method to determine the content of thrombin-like in the snake venom and its extract, which is simple and quick, has less matrix interference, high sensitivity and accurate quantification, and fills the blank of the quality standard of the snake venom and its extract. , improve the quality control level. |