| abstract |
The invention relates to the fields of genetic engineering and antibody preparation, in particular to a nested PCR primer and a kit for amplifying a horse antibody and application thereof. The positive rate of antibody heavy chain and light chain genes matched by the nested PCR primer is about 56%, so that the method for separating the antibody genes from single B lymphocyte can be further used for preparing high-throughput equine antibodies. The method retains the natural pairing of the light chain and the heavy chain variable region, has the advantages of good gene diversity, high titer, good antibody affinity, strong specificity, short experimental period and the like, provides a wide approach for developing preventive and therapeutic drugs or diagnostic antibodies of monoclonal antibodies of infectious diseases and autoimmune diseases, and simultaneously provides a more efficient, simple and rapid method for obtaining antibodies of exogenous proteins for basic research of life science. |