| abstract |
The invention discloses a spinosyn hapten, an artificial antigen and an antibody as well as a preparation method and application thereof, wherein the spinosyn hapten provided by the invention not only retains the characteristic structure of spinosyn to the maximum extent, so that the immunogenicity of the spinosyn hapten is obviously enhanced, but also has carboxyl which can be coupled with carrier protein; the spinosyn artificial antigen obtained by coupling the spinosyn hapten and the carrier protein is used for immunizing animals, so that the method is more favorable for stimulating the immune response of the animals to generate antibodies with stronger specificity and higher sensitivity, the sensitivity of the detected spinosyn antibody can reach 1 mu g/L, the cross reaction rate with other macrolide insecticides is low, and a foundation is provided for the subsequent establishment of various immunoassay methods of the spinosyn. |