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filingDate 2020-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111875560-A
titleOfInvention A ratiometric two-photon fluorescent probe and its preparation method and application
abstract The invention belongs to the technical field of synthesis and fluorescence detection, and discloses a ratio type two-photon fluorescence probe The preparation method and application thereof, the preparation method of the fluorescent probe is: reacting hydroxytetraphenylene with hexamethylenetetramine to obtain compound 1 Compound 1 reacts with o-aminothiophenol to obtain compound 2 Adding trifluoromethanesulfonyl chloride to compound 2 generates the final product, which is the ratio-type two-photon fluorescent probe TPE-BT-TF; the fluorescent probe is applied to the detection of superoxide anions, and the concentration of superoxide anions can be determined , and the selectivity is strong; the fluorescent probe has the characteristic of two-photon excitation, and can perform ratio two-photon fluorescence imaging detection of superoxide anion in samples such as cells by means of two-photon confocal microscope, and the imaging effect is good.
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