http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111855982-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-487 |
| filingDate | 2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111855982-B |
| titleOfInvention | Method for detecting the length of nucleic acid fragments |
| abstract | A method for detecting the length of a nucleic acid fragment, comprising: diluting the nucleic acid to be detected to a predetermined concentration; respectively taking an equal volume of nucleic acid and combining it with magnetic beads of a gradient multiple dosage; adsorbing the magnetic beads bound to the nucleic acid by a magnetic device, and removing the supernatant. Use an equal volume of elution buffer to dissolve the nucleic acid bound to the magnetic beads, and detect the concentration of the dissolved nucleic acid; calculate the concentration difference before and after the binding of the nucleic acid to the magnetic beads, and form the difference between the concentration difference and the amount of the gradient of the magnetic beads. The curve relationship is obtained by comparing the curve relationship with the standard curve to obtain the fragment length of the nucleic acid to be detected, wherein the standard curve includes the concentration difference before and after the combination of nucleic acid standards with different fragment lengths at predetermined concentrations and magnetic beads with gradient multiples and dosages. Correspondence between the amount of magnetic beads gradient multiples. The method of the invention is based on the characteristic of biased screening of nucleic acid fragments with different concentration multiples of the same magnetic beads, and combined with data analysis, the length of nucleic acid fragments can be detected simply and efficiently. |
| priorityDate | 2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.