http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111850029-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
| filingDate | 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111850029-B |
| titleOfInvention | A method for obtaining non-transgenic perennial ryegrass mutants |
| abstract | The invention discloses a method for obtaining a non-transgenic perennial ryegrass mutant. The present invention provides a method for cultivating a target non-transgenic ryegrass mutant, comprising the following steps: 1) suppressing the expression of the ryegrass meiotic protein coding gene LpDMC1 in the perennial ryegrass through the CRISPR/Cas9 system to obtain the transformed ryegrass Plant; 2) PCR/RE experiment was carried out on the transformed ryegrass plant, and the single plant that did not cut the band in the enzyme digestion process and was sequenced as an effective mutation was the mutant ryegrass plant; 3) After the mutation was selected The single plant of ryegrass that does not contain the Cas9 gene is the target non-transgenic ryegrass mutant; the CRISPR/Cas nuclease technology of the present invention obtains a knockout mutant of DMC1, and homologous chromosomes cannot be normally paired and assembled to form a bivalent body In this way, the monovalent body of the sterile plant can be obtained, and the progeny hybridization can form a haploid, which can quickly shorten the breeding period and play a significant role in crop breeding improvement. |
| priorityDate | 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 540.