http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111850016-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2020-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111850016-B |
| titleOfInvention | Immune repertoire standard substance sequence and design method and application thereof |
| abstract | The invention relates to an immune repertoire standard substance sequence and a design method and application thereof, belonging to the technical field of immune repertoire detection. The immune repertoire standard substance sequence sequentially comprises from 5 'end to 3': a V gene reference sequence, a CDR3 mimic sequence, a J gene reference sequence and a C gene reference sequence; the probability of the CDR3 simulation sequence appearing in a real sample is less than or equal to 0.01 percent, and the CDR3 simulation sequence comprises 1-3 sections of identification label sequences and 0-1 section of watermark sequences; the tag sequence is used for identifying and distinguishing the immune repertoire standard substance sequence, and the watermark sequence is used for identifying the source of the immune repertoire standard substance sequence. The sequence is synthesized through artificial design, and is an immune repertoire standard substance for simulating lymphocyte receptor genes, and multiple PCR primer systems can be adjusted and verified by taking the standard substance as a simulation sample, so that the deviation caused by multiple PCR is reduced, and the distribution condition of each clone of the immune repertoire of a reaction sample is more accurate. |
| priorityDate | 2020-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.