| abstract |
The invention discloses an oligo nucleic acid aptamer sequence Seq-32 for recognizing lung cancer serum with high specificity and an application, belonging to the field of molecular biomedicine. The nucleic acid sequence of the oligonucleotide aptamer Seq-32 is shown in SEQ ID NO.1. The present invention designs the Seq-32 specific sequence, and adopts the method of modification and labeling (such as isotope, digoxigenin or biotin, etc.) at the 5' end or the 3' end to be able to specifically identify lung cancer serum. As a detection index of lung cancer serum, Seq-32 is used for auxiliary diagnosis of lung cancer, targeted therapy, or basic research related to the occurrence, development and process of lung cancer. The method of the invention is simple, rapid and sensitive, is suitable for auxiliary diagnosis of preclinical lung cancer and biologically guided treatment of tumors, and has wide clinical application prospect and basic application prospect. |