http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111849861-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5cedae1e4103c4e3719a5e9c94c2b510 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2020-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73ec6465486ac5d33d2fb7d96bd86a4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f58c4807d0142f2cb7714413894ad9d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_049f6ccb80f3a585d730ebd9f9a3b866 |
| publicationDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111849861-A |
| titleOfInvention | A method for separating, extracting and culturing mouse hepatic stellate cells |
| abstract | The invention relates to the field of cell separation, and discloses a method for separating, extracting and culturing mouse hepatic stellate cells. The method includes the following steps: preparing adult mice over 12 weeks old, perfusion to obtain the digested liver in vivo, washing the liver with DMEM with double antibody for multiple times, then mashing the liver, and the mashed liver is treated with collagenase and chain Mycoprotease mixture was digested in a water bath at 37°C for 10 minutes, passed through a 200-mesh filter screen, centrifuged, and the supernatant was removed. After density gradient centrifugation, the hepatic stellate cell medium was placed in 5% CO 2 and 95% humidity. , 37 ℃ incubator, after 24 hours, the medium was changed to remove non-adherent cells, and then the medium was changed every 2 days. The method of the invention is simple and efficient, can obtain mouse hepatic stellate cells in high yield, obtains valuable experimental materials for studying liver fibrosis, and is conducive to fully understanding the role of hepatic stellate cells in the process of liver fibrosis . |
| priorityDate | 2020-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.