http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111848774-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5759 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 |
| filingDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111848774-B |
| titleOfInvention | Preparation method of metreleptin |
| abstract | The invention discloses a preparation method of metreleptin, and particularly relates to a preparation method of metreleptin protein, wherein metreleptin gene is expressed by a prokaryotic expression system to obtain an inclusion body, the inclusion body is dissolved, and then hydrophobic chromatography renaturation is carried out. The dissolution of the inclusion body and the renaturation of the hydrophobic chromatography are carried out in a mild mode, and an antioxidant and/or a metal chelating agent can be added in the process to inhibit the oxidation and degradation of the target protein. The method for preparing the metreleptin is easy to realize industrial amplification, and has high renaturation efficiency, high yield and good biological activity. |
| priorityDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 184.