| abstract |
The invention provides a purification method of total flavonoids of ardisia japonica, and relates to the technical field of traditional Chinese medicine purification. The invention provides a purification method of total flavonoids of ardisia japonica, which comprises the following steps: extracting the ardisia japonica thunb to obtain an extract, concentrating the extract to obtain an ardisia japonica thunb extract, and diluting the ardisia japonica thunb extract with water until the content of the ardisia japonica thunb extract is 1-5 mg/mL to obtain a loading solution; adsorbing the sample solution by using a macroporous resin column, eluting the adsorbed macroporous resin column by using an ethanol solution with the mass concentration of 50-90%, collecting an eluent, concentrating and drying the eluent to obtain the total flavonoids in the ardisia japonica. By utilizing the purification method provided by the invention, the purity of the purified total flavonoids of the ardisia japonica can reach more than 75%. |