http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111808932-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2020-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111808932-B |
| titleOfInvention | Circular RNA detection method and kit |
| abstract | The invention relates to the field of molecular biology, and relates to a circular RNA detection method and a kit, wherein the circular RNA detection method comprises the following steps: s1, designing a primer; s2, reverse transcription reaction: adding N6 Random Primer, RNase-Free H into 0.1-1 μ g of extracted total RNA 2 Mixing O to obtain a reaction solution I; then adding 5 XHT RT Buffer to the reaction solution I, adding HT M-MLV Mix to the reaction solution I, and finally adding RNase-Free H 2 O obtaining cDNA; s3, HS PCR amplification: FSfront printers, 2 XSSNP Taq Mix, 50 XROX Mix, RNase-Free H obtained in step S1 2 And performing qPCR detection on the cDNA obtained in the step S2 and O. |
| priorityDate | 2020-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 549.