http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111808828-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14151 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 |
| filingDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111808828-B |
| titleOfInvention | Separation and purification method of adeno-associated virus |
| abstract | The invention relates to the field of virus separation and purification, in particular to a method for separating and purifying adeno-associated virus. The method comprises the following steps: a) providing a chromatography medium, wherein the chromatography medium comprises a matrix support and an antibody fixed on the matrix support, and the heavy chain variable region sequence and the light chain variable region sequence of the antibody are shown as SEQ ID NO 1 and SEQ ID NO 2 in sequence; b) incubating a liquid phase composition comprising adeno-associated virus with the chromatographic medium such that the adeno-associated virus binds to the antibody; c) eluting the adeno-associated virus from the antibody; wherein the adeno-associated virus is selected from any one or more of AAV 1-13. The method can universally separate and purify any one of AAV1-13, does not need to modify the AAV, and is more convenient to purify the AAV; high purification efficiency, good specificity and almost no detectable non-specific combination with other proteins in cells. |
| priorityDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 167.