http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111781371-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-43578 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2020-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111781371-B |
| titleOfInvention | A method for rapid detection of trace silk fibroin in archaeological sites based on magnetic bead carrier double-antibody sandwich ELISA |
| abstract | The invention relates to the technical field of protein detection, and discloses a method for rapid detection of trace silk fibroin in archaeological sites based on magnetic bead carrier double-antibody sandwich ELISA, comprising the following steps: (1) coupling mouse monoclonal antibody IgA with magnetic beads (2) Add the sample to be tested and incubate to obtain silk fibroin-bound magnetic beads; (3) Add primary antibody and incubate to obtain silk fibroin double-antibody sandwich magnetic beads; (4) Add enzyme label Secondary antibody, incubate to obtain secondary antibody bound to magnetic beads; (5) Add color developing solution for color development; (6) Magnetically separate the supernatant, add stop solution to the supernatant, perform optical density test, and combine with the standard curve , the silk fibroin concentration in the sample to be tested can be obtained. The invention realizes the detection of trace silk fibroin by combining magnetic beads with double-antibody sandwich ELISA, and using mouse monoclonal antibody IgA to prepare immune magnetic beads, and the operation is convenient and time-consuming. |
| priorityDate | 2020-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.