| abstract |
The invention discloses a basic culture medium and a supplemented culture medium required by producing a semaglutide precursor by fermenting escherichia coli, and a method for producing the semaglutide precursor by fermenting the culture medium, aiming at the defects that dissolved oxygen is difficult to control and the supplemented culture speed is slow in the fermentation process of the escherichia coli, so that the fermentation density is low, the expression quantity is low and the like, ferric ammonium citrate and perfluorocarbon emulsion are added in the basic culture, and perfluorocarbon emulsion is added in the supplemented culture medium, so that the dissolved oxygen can be controlled to be more than 30% in the whole fermentation process, and the thallus density and the yield are obviously improved. |