http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111748534-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-13011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2020-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111748534-B |
titleOfInvention | SmC4H protein and construction and expression method thereof |
abstract | The invention provides a SmC4H protein and a construction and expression method thereof, the SmC4H protein utilizes transcriptome sequencing of swertia mussotii Franch, obtains a SmC4H gene sequence through biological information software analysis, obtains a cDNA sequence through RT-PCR amplification, and clones; PBCV-SmC4H is firstly constructed, then an expression vector pETX-SmC4H is constructed, the expression vector is transferred into Escherichia coli Rosetta (DE3) to carry out expression of the protein, the obtained SmC4H protein is purified by a nickel column, and the purification effect is obvious. |
priorityDate | 2020-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.