| abstract |
The invention provides an application of USP33 as a drug target in preparation of drugs, belonging to the field of protein engineering. The invention discovers deubiquitinase USP33 interacting with Parkin by using co-immunoprecipitation and mass spectrometry, and further discovers that USP33 is positioned on the outer membrane of mitochondria and can be used for modifying E3 ligase Parkin by deubiquitination. Knocking down USP33 can promote the translocation of Parkin to mitochondria, thereby enhancing mitophagy and removing damaged mitochondria, and proves that USP33 regulates and controls mitophagy by influencing the deubiquitination of Parkin, USP33 can inhibit the apoptosis of nerve cells under MPTP treatment, USP33 can be used as a medication target to prepare medicines, and has good application potential and value. |