http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111721936-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59b8347ee3db0f210ab505449388e321 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-195 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-579 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-579 |
| filingDate | 2020-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e8179f08a0be712b51ed1be0e797ff1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d234c7000d0262b03f0ab7ed1cfa88d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a71e290858295588e81c3310a46cda10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d638a9790584ffd45d282efb50312c84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b07e4f7e8da9c25d3b15b50576ce3dc |
| publicationDate | 2020-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111721936-A |
| titleOfInvention | A kind of detection method of bacterial endotoxin in nalbuphine hydrochloride crude drug |
| abstract | The invention belongs to the technical field of microbial testing, and in particular relates to a method for detecting bacterial endotoxin in a nalbuphine hydrochloride bulk drug. The steps are as follows: (1) determining the bacterial endotoxin limit L: the bacterial endotoxin in the injection is limited to Considering the safety of later clinical use, the bacterial endotoxin limit was determined as L=2EU/mg; (2) To check the sensitivity of Limulus Reagent: the bacterial endotoxin standard with concentrations of 2λ, λ, 0.5λ and 0.25λ was used Check the sensitivity of the Limulus reagent and record the results; (3) Pre-experiment of the interference test, determine that the maximum effective dilution factor is 80 times; (4) The interference test of the test substance, determine that the non-interference dilution factor is 160 times; (5) Detect the supply The content of bacterial endotoxin in the test product: take the test product and prepare a 10 mg/ml stock solution, gradually dilute it to 160 times to obtain a dilution, and then dilute the dilution by 1, 2, 4, and 8 times to obtain The test solution is respectively reacted with the Limulus reagent, and the content of endotoxin in the test is quantified by determining the reaction concentration. |
| priorityDate | 2020-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.