http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111718905-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dd44b7a6402701f204eb7540856d1d95 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52d94ed35388b70f8b7eb70693952072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd28517070937f70923ef8f3c7d7c6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_542c2766f8a995803514fa42a39d83f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1448c7e6e3dc584e458178a66d710f0 |
| publicationDate | 2020-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111718905-A |
| titleOfInvention | Human type Ⅱ thrombocytopenia iPS cell line and preparation method and application thereof |
| abstract | The invention relates to the field of biotechnology, in particular to a human type II thrombocytopenia iPS cell line and a preparation method and application thereof. Transcription factors are introduced into CD34 + cells of patients with type II hereditary thrombocytopenia, and the cells are reprogrammed to form; the preparation method includes isolating mononuclear cells from isolated bone marrow specimens, sorting to obtain CD34 + cells, and carrying out analysis of CD34 + cells. Cell culture; use viral vectors containing Oct4, Sox2, Klf4 and c-Myc genes to transfect cultured CD34 + cells, then centrifuge, carry out cell culture to reprogram the CD34 + cells, and select successfully reprogrammed cells to obtain iPS cell line. The invention reprograms the CD34 + cells derived from the bone marrow of type II thrombocytopenia patients with c.-128G>T heterozygous mutation in the 5'-UTR region of the Ankerd26 gene into an iPS cell line, and the prepared iPS cell line maintains a long life and can be It is used in the establishment of in vitro disease models and drug screening of type Ⅱ thrombocytopenia. |
| priorityDate | 2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 233.