http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111705108-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3966ddee295e08cc6fcce4695c77f91b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-942 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
| filingDate | 2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06e5b0450d21febf7e505015c2a9cb8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_652700b9922fdc676232729d290188b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87e3644d26570d4886706b189418cf4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ab12b589bad5e75a3b65b2a569678e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de754a86a8ec868d9617ef2108377431 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0457f7da05a74eb2ff177c73030dcb9a |
| publicationDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111705108-A |
| titleOfInvention | A kind of method for detecting β-glucosidase activity of litter |
| abstract | The invention discloses a method for detecting β-glucosidase activity of litter, belonging to the technical field of biochemistry. The main steps of the method for detecting β-glucosidase activity in litters provided by the present invention are as follows: weighing the shredded and sieved litters into a centrifugal filter tube, adding a culture buffer for incubation, mixing the supernatant after high-speed centrifugation, Adjust to a certain volume as the enzyme activity test solution; add the enzyme activity test solution and the inactivated enzyme activity test solution to the sample activity well and negative control well, respectively, and then add the fluorescent substrate working solution 4‑MUB‑β‑D -Glucoside solution, incubate with shaking in the dark; then add Tris stop solution to the microplate wells; use a multi-function microplate reader for fluorescence detection; calculate the β-glucosidase activity. The advantages of the present invention are: after the reaction system is optimized, the sensitivity is high, the detection consumption of the enzyme liquid to be tested is small, the operation time is shortened, the measurement data can be obtained in large quantities in a short time, the operation is simple, and the efficiency is high. |
| priorityDate | 2020-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.