http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111705079-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2999-007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2020-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111705079-B |
| titleOfInvention | Gene editing method based on Vcre-Vloxp recombinase system and application thereof |
| abstract | The invention provides a gene editing method based on a Vcre-Vloxp recombinase system and application thereof, wherein the method comprises the following steps: (1) ligating a Vloxp sequence to the 5 'end or 3' end of the double-stranded DNA, or inserting said Vloxp sequence between functional regions of the double-stranded DNA; (2) and then mixing the double-stranded DNA, the Vcre protein and the reaction solution for gene editing, and transferring the mixture into a target cell to finish the gene editing. When the phenomenon of repeated connection of double-stranded DNA occurs in the integration process, the double-stranded DNA is sheared under the action of Vcre protein, only one double-stranded DNA is finally left, and no matter how many double-stranded DNAs are connected in series, the Vcre protein can cut all the repeated fragments from the first Vloxp sequence to the last Vloxp sequence, so that the method can obviously reduce the phenomenon of repeated connection of recombinant DNA fragments in the integration process. |
| priorityDate | 2020-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.