http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111705044-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ac31afbea1cbbb03498644721ffb4a62 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2020-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d346e0a716ebe0153d0cdd6693b6ddf7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_395ef7dcbd4a8a1f0a32d8b8c743774a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f9cfb490c18ece02e5be68b16d548de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2207603b22ff2066701df8b061eb38e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a012353797bf5878ccdb6244c221a52 |
| publicationDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111705044-A |
| titleOfInvention | Construction of a novel controllable and highly active G-quadruplex DNase |
| abstract | The invention relates to the construction of a meta-cytosine-enhanced and single-base modification technology to further realize the construction of a G-quadruplex DNAase with high catalytic activity. First, cytosine and its derivatives were modified at the 3' end of the G base-rich DNA sequence, and annealed at 95 °C for 5 min in a buffer containing 100 mM K ions to form a G-quadruplex structure. The G-quadruplex is then mixed with hemin to form DNase. The Michaelis constants of different derivatives were obtained by testing the catalytic activity of DNase at different concentrations of H 2 O 2 and fitting with the Michaelis model. Finally, when the 3'-terminal meta-base is azacytidine, its activity reaches the same order of magnitude as that of the native protease HRP. The high-activity G-quadruplex DNase is simple in design, fast in preparation, low in price, convenient in storage, and has certain application prospects. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114591983-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114591983-A |
| priorityDate | 2020-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.