http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111705032-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-02007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111705032-B |
| titleOfInvention | Prokaryotic expression cell and preparation method and application thereof |
| abstract | The embodiment of the invention relates to a prokaryotic expression cell and a preparation method and application thereof, wherein the prokaryotic expression cell can co-express heparinase I HepA' and chaperone CpkA. In the amino acid sequence of heparinase I HepA', the key is to replace the leader peptide amino acid sequence in HepA with a signal tag peptide with net positive charge. The expression quantity of the modified heparinase I HepA' containing a section of signal tag peptide with net positive charges in prokaryotic expression cells is greatly improved, and particularly the quantity of protein existing in supernate is greatly improved; meanwhile, the carboxyl end of the CpkA has net negative charge and generates electrostatic interaction with the signal tag peptide with net positive charge, so that the binding force of the CpkA and HepA 'is stronger, and the CpkA can more effectively help the HepA' to fold into a correct conformation, thereby improving the soluble expression of the CpkA. |
| priorityDate | 2020-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.