| abstract |
The invention discloses an ultra-sensitive protein detection method based on DNA signal amplification and blood glucose meter. The product of the target molecule amplified by the DNA signal can undergo base complementary pairing with the DNA sequence modified by sucrose oxidase, thereby catalyzing the hydrolysis of sucrose into glucose, Finally, it is detected by a commercial blood glucose meter; then combined with a microfluidic system, the capture antibody is modified on the ultra-thin glass, which has good reproducibility, sensitivity and specificity, and is of great significance for the rapid and low-cost detection of target proteins. |