http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111679081-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80babc6a23956646d6fafc412552c167 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-912 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-71 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57438 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5748 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2020-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c26020aa668fffd13ec6b09c518d8351 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1da1867f8a92db4abf72b204b5483aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19cb922bf4c10775e29d4631237e379f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f296b4323c4d60fd7855b98cc14a89b1 |
| publicationDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111679081-A |
| titleOfInvention | An immunofluorescence kit and detection method for detecting the expression of C-MET in peripheral blood circulating tumor cells of patients with hepatocellular carcinoma |
| abstract | The invention discloses an immunofluorescence detection method for C-MET expression on peripheral blood circulating tumor cells of patients with hepatocellular carcinoma, comprising 8% PFA, 0.4% PFA, 0.3% TritonX-100, 10% goat serum, rabbit anti-human C-MET , 100 μl of primary antibody suspension composed of rat anti-CD45, 100 μl of secondary antibody suspension composed of fluorescently labeled goat anti-rabbit and fluorescently labeled goat anti-rat, DAPI mounting medium; detection methods include peripheral blood collection, peripheral blood Blood sample pretreatment, sample separation, methanol fixation, film sticking, histochemical stroke circle, cell fixation, cell penetration, blocking, primary antibody incubation, secondary antibody incubation and nuclear staining, mounting and detection. The detection method provided by the present invention can detect the expression of C-MET in patients with advanced or recurrent hepatocellular carcinoma without obtaining tissue samples by needle biopsy, and can realize real-time dynamic detection by using a minimally invasive technology. |
| priorityDate | 2020-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 147.