http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111679069-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d56092b43d7803f8413fd92d8852109d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-40 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56961 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52c4eb598663c98ea57175bc0508eb19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f481ed0212c2140d63c4776b991d4ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a94bbb5ac44be3c0ef0186dcee02909 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d762e76f5105fe47059562089a8944da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_20a5ea28bfbf23c1f2adf73b3e16cf89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cede94b93492178a290d4e9a48c26e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c2520e4f98373fd3cf03a9637427667 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64c2f257c368292e4b82a5c1b4202d8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86e11eeeaee842aa9b89f43421bc3136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9ed06a1897a2f96a53898dd67f37e92 |
| publicationDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111679069-A |
| titleOfInvention | A method for evaluating the antifungal effect of traditional Chinese medicine monomers by remodeling of Candida auris cell wall |
| abstract | A method for evaluating the antifungal effect of a traditional Chinese medicine monomer by remodeling the cell wall of Candida auris, comprising adding the traditional Chinese medicine monomer to the evaluation bacterial solution, after incubating for 12 hours, washing once with sterile PBS, and then preparing the quality of the product with sterile PBS. BSA with a fraction of 2% was blocked for 1 hour, and then washed once with sterile PBS; next, 100ul of β-glucan antibody was added, placed in a 4°C refrigerator for staining for 4 hours, and after staining, washed with sterile PBS 2 times, and then stained with cy3 monoclonal antibody mouse IgG for 1 hour at room temperature in the dark, then washed 3 times with sterile PBS in the dark, resuspended in 1 ml of sterile PBS in the dark, shaken in the dark, and mixed with light. The fluorescence exposure intensity of glucan was detected by flow cytometer; or the fluorescence exposure intensity of chitin was detected by flow cytometer; or the fluorescence images of β-glucan and chitin were recorded by inverted fluorescence microscope. The invention utilizes Candida auris cell wall remodeling to evaluate the antifungal effect of the traditional Chinese medicine monomer, and has clinical significance. |
| priorityDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.