| abstract |
The invention discloses a fluorescent detection method, primers and probes for rapidly distinguishing African swine fever virus genotype II from other genotypes. The method combines real-time fluorescent PCR technology and melting curve analysis technology, and identifies African swine fever virus genotype II and other genotypes according to the difference in the Tm value of the melting curve; the operation is simple, the detection speed is fast and the throughput is high, and the detection method of the invention is accurate It has high performance, good specificity, and good repeatability, and can be analyzed accurately, rapidly, and with high throughput, which is beneficial to popularization and application in clinical practice. |