http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111676210-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
filingDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111676210-B |
titleOfInvention | Method for improving cellulase activity, cellulase mutant 5I77-M and application |
abstract | The invention relates to the technical field of agricultural biology, in particular to a method for improving cellulase activity, a cellulase mutant 5I77-M and application. The invention relates to a polypeptide with an amino acid sequence shown as SEQ ID NO: site-directed mutagenesis is carried out on the T300/D307 sites of the wild-type cellulase shown in the 1 to obtain a T300P/D307P mutant. The results show that compared with the wild cellulase, the optimum pH value and the optimum temperature of the mutant are not changed, and when the sodium carboxymethyl cellulose is used as a substrate, the specific activity of the mutant is improved by about 60 percent compared with the wild cellulase. |
priorityDate | 2020-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.